Dear All, The CEU Department of Cognitive Science and the Center for Cognitive Computation (CCC) invites you to the upcoming event of the Budapest Computational Neuroscience Forum<https://ccc.ceu.edu/budapest-computational-neuroscience-forum>um>. Speaker: Zoltán Somogyvári, Wigner Research Centre for Physics Seeing beyond the spikes: reconstructing the complete spatiotemporal membrane potential distribution from paired intra- and extracellular recordings Abstract: Although electrophysiologists have been recording intracellular neural activity routinely ever since the ground-breaking work of Hodgkin and Huxley, and extracellular multichannel electrodes have also been used frequently and extensively, a practical experimental method to track changes in membrane potential along a complete single neuron is still lacking. Instead of obtaining multiple intracellular measurements on the same neuron, we propose an alternative method by combining single-channel somatic patch-clamp and multichannel extracellular potential recordings. In this work, we show that it is possible to reconstruct the complete spatiotemporal distribution of the membrane potential of a single neuron with the spatial resolution of an extracellular probe during action potential generation. Moreover, the reconstruction of the membrane potential allows us to distinguish between the two major but previously hidden components of the current source density (CSD) distribution: the resistive and the capacitive currents. This distinction provides a clue to the clear interpretation of the CSD analysis, because the resistive component corresponds to transmembrane ionic currents (all the synaptic, voltage-sensitive and passive currents), whereas capacitive currents are considered to be the main contributors of counter-currents. We validate our model-based reconstruction approach on simulations and demonstrate its application to experimental data obtained in vitro via paired extracellular and intracellular recordings from a single pyramidal cell of the rat hippocampus. In perspective, the estimation of the spatial distribution of resistive membrane currents makes it possible to distinguish between active and passive sinks and sources of the CSD map and the localization of the synaptic input currents, which make the neuron fire. Time: 17:00, Wednesday January 31., 2024. Location: CEU Budapest (1051 Budapest, Nádor u. 15.) N15. room 101. Zoom: Meeting ID: 918 8554 1295<https://ceu-edu.zoom.us/j/91885541295?pwd=SlhyVFdKZDc3Y2N2VGh2WllYc01Fdz09> Passcode: 141200 Should you have any inquiries about the series, please contact Mihály Bányai<mailto:mihaly.s.banyai@gmail.com>. Please, be informed that video/photo recording might take place at the event and the edited version of the video material might be published to communicate or promote CEU's activities. Please, find our Privacy Notice here<https://www.ceu.edu/privacy>cy>. Best regards, Ildikó Varga Department Coordinator (Budapest) Department of Cognitive Science [cid:f43aa0cb-db14-4a5e-85e6-067b401f9e55] H-1051 Budapest Nádor u. 15. FT room 404. tel: +36-1 327-3000 2941 http://www.ceu.edu<http://www.ceu.edu/> http://cognitivescience.ceu.edu<http://cognitivescience.ceu.edu/> ________________________________ From: Ildiko Zsoka Varga Sent: Thursday, January 25, 2024 3:03 PM To: &#39;talks(a)cogsci.ceu.edu (talks(a)cogsci.ceu.edu)&#39; &lt;talks(a)cogsci.ceu.edu&gt; Subject: Budapest Computational Neuroscience Forum - Jan 31. Dear All, The CEU Department of Cognitive Science and the Center for Cognitive Computation (CCC) invites you to the upcoming event of the Budapest Computational Neuroscience Forum<https://ccc.ceu.edu/budapest-computational-neuroscience-forum>um>. Speaker: Zoltán Somogyvári, Wigner Research Centre for Physics Seeing beyond the spikes: reconstructing the complete spatiotemporal membrane potential distribution from paired intra- and extracellular recordings Abstract: Although electrophysiologists have been recording intracellular neural activity routinely ever since the ground-breaking work of Hodgkin and Huxley, and extracellular multichannel electrodes have also been used frequently and extensively, a practical experimental method to track changes in membrane potential along a complete single neuron is still lacking. Instead of obtaining multiple intracellular measurements on the same neuron, we propose an alternative method by combining single-channel somatic patch-clamp and multichannel extracellular potential recordings. In this work, we show that it is possible to reconstruct the complete spatiotemporal distribution of the membrane potential of a single neuron with the spatial resolution of an extracellular probe during action potential generation. Moreover, the reconstruction of the membrane potential allows us to distinguish between the two major but previously hidden components of the current source density (CSD) distribution: the resistive and the capacitive currents. This distinction provides a clue to the clear interpretation of the CSD analysis, because the resistive component corresponds to transmembrane ionic currents (all the synaptic, voltage-sensitive and passive currents), whereas capacitive currents are considered to be the main contributors of counter-currents. We validate our model-based reconstruction approach on simulations and demonstrate its application to experimental data obtained in vitro via paired extracellular and intracellular recordings from a single pyramidal cell of the rat hippocampus. In perspective, the estimation of the spatial distribution of resistive membrane currents makes it possible to distinguish between active and passive sinks and sources of the CSD map and the localization of the synaptic input currents, which make the neuron fire. Time: 17:00, Wednesday January 31., 2024. Location: CEU Budapest (1051 Budapest, Nádor u. 15.) N15. room 101. Zoom: Meeting ID: 918 8554 1295<https://ceu-edu.zoom.us/j/91885541295?pwd=SlhyVFdKZDc3Y2N2VGh2WllYc01Fdz09> Passcode: 141200 Should you have any inquiries about the series, please contact Mihály Bányai<mailto:mihaly.s.banyai@gmail.com>. Please, be informed that video/photo recording might take place at the event and the edited version of the video material might be published to communicate or promote CEU's activities. Please, find our Privacy Notice here<https://www.ceu.edu/privacy>cy>. Best regards, Ildikó Varga Department Coordinator (Budapest) Department of Cognitive Science [cid:6e4411db-f547-43cd-b375-6f1f035e3131] H-1051 Budapest Nador u. 15. FT room 404 tel: +36-1 327-3000 2941 http://www.ceu.edu<http://www.ceu.edu/> http://cognitivescience.ceu.edu<http://cognitivescience.ceu.edu/> ______________________________________________ Subscribe by sending an empty mail to talks-subscribe(a)cogsci.ceu.edu Unsubscribe by sending an empty mail to talks-unsubscribe(a)cogsci.ceu.edu